Journal: Experimental and Therapeutic Medicine

Article Title: Tilianin attenuates MPP + -induced oxidative stress and apoptosis of dopaminergic neurons in a cellular model of Parkinson's disease

doi: 10.3892/etm.2022.11223

Figure Lengend Snippet: Effects of tilianin on the viability of MES23.5 cells. (A) Chemical structure of tilianin. (B) Effects of tilianin at different concentrations on cell viability were examined using the Cell Counting Kit-8 assay. Results are presented as the mean ± SD.

Article Snippet: The dopaminergic neuron MES23.5 cell line (cat. no. CVCL-J351; http://www.biovector.net/product/2277098.html ) was acquired from the BioVector National Type Culture Collection, Inc. MES23.5 cells were maintained in DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Merck KGaA), 2% Sato's solution containing 25 mg insulin, transferrin, selenium and sodium pyruvate solution (ITS-A; cat. no. 51300044; Thermo Fisher Scientific, Inc.), 0.315 mg/ml progesterone (cat. no. HY-N0437; MedChemExpress) and 20 mg putrescine (cat. no. HY-N2407; MedChemExpress), in addition to 100 U/ml penicillin/streptomycin in a humidified atmosphere containing 5% CO 2 at 37˚C.

Techniques: Cell Counting

Journal: Experimental and Therapeutic Medicine

Article Title: Tilianin attenuates MPP + -induced oxidative stress and apoptosis of dopaminergic neurons in a cellular model of Parkinson's disease

doi: 10.3892/etm.2022.11223

Figure Lengend Snippet: Effects of tilianin on the MAPK pathway in MPP + -stimulated MES23.5 cells. The expression levels of p-p38MAPK, p38MAPK, p-ERK1/2, ERK1/2, p-JNK and JNK in cells stimulated with MPP + with or without tilianin preconditioning were detected by western blotting. Results are presented as the mean ± SD. ** P<0.01, *** P<0.001. MPP + , 1-methyl-4-phenylpyridinium; p, phosphorylated.

Article Snippet: The dopaminergic neuron MES23.5 cell line (cat. no. CVCL-J351; http://www.biovector.net/product/2277098.html ) was acquired from the BioVector National Type Culture Collection, Inc. MES23.5 cells were maintained in DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Merck KGaA), 2% Sato's solution containing 25 mg insulin, transferrin, selenium and sodium pyruvate solution (ITS-A; cat. no. 51300044; Thermo Fisher Scientific, Inc.), 0.315 mg/ml progesterone (cat. no. HY-N0437; MedChemExpress) and 20 mg putrescine (cat. no. HY-N2407; MedChemExpress), in addition to 100 U/ml penicillin/streptomycin in a humidified atmosphere containing 5% CO 2 at 37˚C.

Techniques: Expressing, Western Blot